Anticancer Activity Test of Bamboo Grass (Lophatherum gracile B.) Extract Using Ultrasonic Method Against T47D Breast Cancer Cells
Abstract
Bamboo grass (Lophatherum gracile B.) is a type of weed that can be utilized as a medicinal plant. Almost all parts of this plant contain secondary metabolites with potential as herbal medicine. This study was conducted to evaluate the breast cancer (T47D) anticancer activity of bamboo grass using an ultrasonic extraction method. The solvents used in this study were 80% ethanol, n-hexane, and chloroform. All extract samples were tested for their breast cancer (T47D) anticancer activity using the MTT assay. The IC50 values of the 80% ethanol, n-hexane, and chloroform extracts were 218.081; 292.412; and 395.144 ?g/mL, respectively. The TLC separation of tannin compounds from the 80% ethanol extract was identified using FTIR. The identification results showed key absorption peaks of tannin compounds, including O-H alcohol, C-O secondary alcohol, aliphatic C-H, C=C alkene, and ortho-substituted aromatic ring, with corresponding wavenumbers of 3455; 1094; 2963; 2929; 1650; and 780 cm?¹.References
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Sari, S. P. (2014). Cytotoxic activity of crude extract of bamboo grass leaves (Lophatherum gracile B.) against Artemia salina Leach shrimp larvae and identification of its active compounds. Maulana Malik Ibrahim State Islamic Univ. of Malang,.
Wulandari, E. (2017). Uji aktivitas ekstrak kasar etanol dan fraksi n-heksana tanaman rumput bambu (Lophatherum gracile B.) sebagai antimalaria pada parasit Plasmodium falciparum strain 3D7. Universitas Islam Negeri Maulana Malik Ibrahim Malang.
Yao, B., Zhang, M., Zhao, S., Yu, H., Zhang, J., & Liu, D. (2025). Research and utilization status of Lophatherum gracile: A medicinal and food homologous plant. Chinese Herbal Medicines, 17(2), 261–278. https://doi.org/10.1016/j.chmed.2024.07.006
Zhang, P., Chun, Z., Shao, Q., Fu, L., Luo, Y., Gu, D., & Chen, R. (2021). Evaluation of the phytochemicals and antioxidant activity of Lophatherum gracile Brongn based on chemical fingerprinting by HPLC with electrochemical detection. Journal of Separation Science, 44(20), 3777–3788. https://doi.org/10.1002/jssc.202100318
Asma, S. T., Acaroz, U., Imre, K., Morar, A., Shah, S. R. A., Hussain, S. Z., Arslan-Acaroz, D., Demirbas, H., Hajrulai-Musliu, Z., Istanbullugil, F. R., Soleimanzadeh, A., Morozov, D., Zhu, K., Herman, V., Ayad, A., Athanassiou, C., & Ince, S. (2022). Natural Products/Bioactive Compounds as a Source of Anticancer Drugs. Cancers, 14(24), 6203. https://doi.org/10.3390/cancers14246203
Auwaliyah, F. (2015). Antioxidant activity test of bamboo grass leaf extract (Lophatherum gracile Brongn) using DPPH method and identification of its active compounds. Maulana Malik Ibrahim State Islamic Univ. of Malang.
Brown, J. S., Amend, S. R., Austin, R. H., Gatenby, R. A., Hammarlund, E. U., & Pienta, K. J. (2023). Updating the Definition of Cancer. Molecular Cancer Research, 21(11), 1142–1147. https://doi.org/10.1158/1541-7786.MCR-23-0411
Bucur, M.-P., Radulescu, M.-C., Radu, G. L., & Bucur, B. (2023). Cavitation-Effect-Based Treatments and Extractions for Superior Fruit and Milk Valorisation. Molecules, 28(12), 4677. https://doi.org/10.3390/molecules28124677
Chen, Y.-L., Chen, C.-Y., Lai, K.-H., Chang, Y.-C., & Hwang, T.-L. (2023). Anti-inflammatory and antiviral activities of flavone C-glycosides of Lophatherum gracile for COVID-19. Journal of Functional Foods, 101, 105407. https://doi.org/10.1016/j.jff.2023.105407
Debiasi, B. W., Rodrigues, P. G. R. S., Torres, M. P. R., Bonacorsi, C., Andrighetti, C. R., Ribeiro, E. B., & Valladão, D. M. S. (2021). Comparison between maceration and ultrasound-assisted extraction of white bracts with flowers of Bougainvillea spectabilis Willd. Scientific Electronic Archives, 14(2), 47. https://doi.org/10.36560/14220211297
Deng, Y., Huang, D., & Yue, T. (2021). Purification Technology of Polysaccharides from Lophatherum gracile Brongn. by Macroporous Resin Adsorption and Its Effect on Athletic Endurance of Mice. Science and Technology of Food Industry, 42(14), 169–174. https://doi.org/10.13386/j.issn1002-0306.2020090194
Firdaus, I. A. (2016). Identifikasi tanin pada fraksi air tanaman rumput bambu (Lophatherum gracile B.) dan uji aktivitas antikanker isolat tanin terhadap sel kanker payudara T47D. Maulana Malik Ibrahim State Islamic University of Malang.
Firdaus, S. M., Permadi, A., & Sulistiawati, E. (2025). Comparative study of ultrasonic and maceration extraction in enhancing antioxidant and SPF properties of green coffee beans serum. Current Research on Biosciences and Biotechnology, 6(2), 2025–2061. https://doi.org/10.5614/crbb.2025.6.2/GAMCE9IN
Istiqomah, A., Muti’ah, R., & Hayati, E. K. (2015). Anticancer Activity against Breast Cancer Cells T47D and Identification of Its Compound from Extracts and Fractions of Leaves Bamboo Grass (Lophaterum gracile B.). ALCHEMY, 4(1). https://doi.org/10.18860/al.v4i1.3138
Lai, K.-H., Chen, P.-J., Chen, C.-C., Yang, S.-H., El-Shazly, M., Chang, Y.-C., Wu, Y.-H., Wu, Y.-H., Wang, Y.-H., Hsieh, H.-L., & Hwang, T.-L. (2021). Lophatherum gracile Brongn. attenuates neutrophilic inflammation through inhibition of JNK and calcium. Journal of Ethnopharmacology, 264, 113224. https://doi.org/10.1016/j.jep.2020.113224
Lee, S.-J., Jang, S.-A., Kim, S. C., Ryuk, J. A., & Ha, H. (2022). Lophatherum gracile Bronghiart Suppresses Receptor Activator of Nuclear Factor Kappa-B Ligand-Stimulated Osteoclastogenesis and Prevents Ovariectomy-Induced Osteoporosis. International Journal of Molecular Sciences, 23(22), 13942. https://doi.org/10.3390/ijms232213942
Liu, X., Wang, Y., Ge, W., Cai, G., Guo, Y., & Gong, J. (2022). Spectrum–effect relationship between ultra?high?performance liquid chromatography fingerprints and antioxidant activities of Lophatherum gracile Brongn. Food Science & Nutrition, 10(5), 1592–1601. https://doi.org/10.1002/fsn3.2782
Raj?evi?, N., Bukvi?ki, D., Dodoš, T., & Marin, P. D. (2022). Interactions between Natural Products—A Review. Metabolites, 12(12), 1256. https://doi.org/10.3390/metabo12121256
Ruiz-Aquino, F., Feria-Reyes, R., Rutiaga-Quiñones, J. G., Robledo-Taboada, L. H., & Gabriel-Parra, R. (2023). Characterization of tannin extracts derived from the bark of four tree species by HPLC and FTIR. Forest Science and Technology, 19(1), 38–46. https://doi.org/10.1080/21580103.2023.2166593
Sari, S. P. (2014). Cytotoxic activity of crude extract of bamboo grass leaves (Lophatherum gracile B.) against Artemia salina Leach shrimp larvae and identification of its active compounds. Maulana Malik Ibrahim State Islamic Univ. of Malang,.
Wulandari, E. (2017). Uji aktivitas ekstrak kasar etanol dan fraksi n-heksana tanaman rumput bambu (Lophatherum gracile B.) sebagai antimalaria pada parasit Plasmodium falciparum strain 3D7. Universitas Islam Negeri Maulana Malik Ibrahim Malang.
Yao, B., Zhang, M., Zhao, S., Yu, H., Zhang, J., & Liu, D. (2025). Research and utilization status of Lophatherum gracile: A medicinal and food homologous plant. Chinese Herbal Medicines, 17(2), 261–278. https://doi.org/10.1016/j.chmed.2024.07.006
Zhang, P., Chun, Z., Shao, Q., Fu, L., Luo, Y., Gu, D., & Chen, R. (2021). Evaluation of the phytochemicals and antioxidant activity of Lophatherum gracile Brongn based on chemical fingerprinting by HPLC with electrochemical detection. Journal of Separation Science, 44(20), 3777–3788. https://doi.org/10.1002/jssc.202100318
Published
2025-08-24
How to Cite
A'YUN, Qurrotul et al.
Anticancer Activity Test of Bamboo Grass (Lophatherum gracile B.) Extract Using Ultrasonic Method Against T47D Breast Cancer Cells.
Jurnal Borneo Cendekia, [S.l.], v. 9, n. 1, p. 55-68, aug. 2025.
ISSN 2549-1822.
Available at: <https://journal.stikesborneocendekiamedika.ac.id/index.php/jbc/article/view/629>. Date accessed: 12 dec. 2025.
doi: https://doi.org/10.54411/jbc.v9i1.629.
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